we develop

Technological

units

 

for the development of advanced and pharmaceutical technologies
and for cutting-edge translational and preclinical research infrastructure.

GMP units for ATMP production

GMP units are certified clean rooms for the production of advanced therapies (ATMPs) for translational research and early phase clinical trials.
As a national ATMP HUB, they strengthen the Czech Republic’s capacity for innovative therapeutic approaches and enable the implementation of high quality, often multi-national ATMP clinical trials.

ATMP production in a GMP certified environment

Technology for storage and transport of ATMP products

Development, optimization and scale-up of processes for the early stages of ATMP development

Processes for decentralised GMP production

GMP units involved

The GMP Unit of UHKT has GMP certification for the production and quality control of advanced and gene therapy medicines at the EU level, including aseptic production and specialized laboratory methods.
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The cGMP Unit, FNUSA | Cell and Tissue Engineering Facility (CTEF-cGMP) focuses on the production and quality control of advanced drug products according to GMP standards for preclinical and clinical studies.
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ACIU Unit LF MU is an academic GMP unit approved by the State Institute for Drug Control (SÚKL) for the development and production of advanced therapies including cellular products (ATMP), with an emphasis on the translation of academic discoveries into clinical research.
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Key laboratory equipment

VVI CZECRIN has specialised instrumentation and technological facilities for the development, production and control of advanced therapeutics (ATMP) as well as for advanced preclinical and translational research.

ACIU Unit LF MU

Development&GMP Manufacturing of Cell Therapies

  • Laminar flow box class II (biohazard)
  • Elutra Cell Separation System (separator of monocyte fraction from leukapheresis product)
  • Inverted microscope, photometers, CO2 incubators
  • Cytometer MACSQuant 10
  • Light Cycler (real-time PCR)
  • Flow cytometers

cGMP FN USA

Development&GMP Manufacturing of Cell&Gene Therapies

  • Independent systems for cell expansion: CliniMACS Prodigy®
  • Inverted Fluorescence Microscope with micromanipulators and injectors for applications such as Confocal
  • High Content Analysis (HCS), and Photobleaching/ Photo Activation to study interaction of fluorescence protein molecules in living cells and tissues
  • System; Terumo Quantum® Cell Expansion System
  • Cryobank

GMP Unit UHKT

Development&GMP Manufacturing of Cell&Gene Therapies

  • BD FACSDiscover S8 cell sorter

Bioanalytical Laboratory

Monitoring of the metabolic activity of selective substrates

  • Evaporator
  • HPLC system
  • LC-MS – Liquid Chromatography-Mass Spectrometry

Laboratory of Protein Engineering

Molecular cloning and high-throughput protein expression screening

  • Flow-box
  • Centrifuge

Molecular Pathophysiology Laboratory

Real-time deformability cytometry (RT-DC) and high-speed video-microscopy

  • Automated cell counter
  • Cell deformability cytometer
  • Fluorometer

Advanced Human Genomic Laboratory

Whole-exome (WES) and whole-genome sequencing (WGS), genetic modifications (CRISPR/Cas9, shRNA, RNA interference, plasmids, overexpression systems)

  • Native long-read sequencing (PacBIO Revio)
  • RNAseq

PharmTech Laboratories

PharmTech Labs are multidisciplinary research units focused on chemical and cellular pharmacology. They integrate expertise in bioanalytical methods, cellular and molecular pathophysiology, advanced genomics and protein engineering.
Under the OP JAK PHARMTECH project, they have undergone a major upgrade and provide cutting-edge analytical and biotechnological tools for preclinical and translational research.

Technology for protein characterisation and engineering

Advanced bioanalytical technologies

Methods of cellular and molecular pathophysiology

Advanced genomic analysis

Laboratories involved

Advanced Human Genomics Lab

Bioanalytical
Lab

Molecular Pathophysiology Lab

Lab of Protein Engineering